Enzymes of the Tricarboxylic Acid Cycle in Acetic Acid Bacteria

Tricarboxylic Acid Cycle in

Tricarboxylic acid cycle enzymes of the ectomycorrhizal basidiomycete, Suillus bovinus.

In crude cell extracts of the ectomycorrhizal fungus, Suillus bovinus, activities of citrate synthase, aconitase, isocitrate dehydrogenase, succinate dehydrogenase, fumarase, and malate dehydrogenase have been proved and analyzed. Citrate synthase exhibited high affinities for both its substrates: oxaloacetate (Km = 0.018 mM) and acetyl-CoA (Km = 0.014 mM). Aconitase showed better affinity for ...

Intracellular distribution of tricarboxylic acid cycle enzymes in Azotobacter vinelandii.

The cytological complexity of the bacterial cell is well established, but little information is as yet available on the association of metabolic function with cellular structure in microorganisms. The significance of the tricarboxylic acid (TCA) cycle and the electron transport system in the metabolism of the obligate aerobic azotobacter cell makes important a knowledge of the intracellular dis...

Acetic acid oxidation by Escherichia coli; quantitative significance of the tricarboxylic acid cycle.

Krebs (1937) reported that acetate and a number of other substrates were oxidized under anaerobic conditions by nonproliferating suspensions of Eschrichia coli when fumarate was added as an oxidant. These observations resulted from a study of the role of fumarate in respiration, and no consideration was given to the mechanism of acetate oxidation. The over-all reaction for the oxidation of acet...

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of 02 utilization was also determined over differe...